나노바디 & 항체 연구실 | 연세대학교 KJY lab

Choi, A. Y., Kim, D., Lee, C., Ahn, J. S., Yang, D. H., Lee, D., & Kim, J. Y. (2026). A novel anti-CD20, concabody, enhances immunotherapy efficacy by targeting MPZL1 and augmenting antibody-induced cell death. Frontiers in Oncology, 16, 1748576. https://doi.org/10.3389/fonc.2026.1748576
Oh, J., Jin, N., Kwon, S., Lee, C., Baek, H., Lee, D., & Kim, J.Y.* (2026). Obinutuzumab induces lysosomal destabilization via sphingomyelin-dependent inhibition of TRPML2. Scientific Reports. https://doi.org/10.1038/s41598-026-38087-5
Kim, Y., Oh, J., Kim, J. R., Lee, D., & Kim, J. Y.* (2025). CRISPRi screening identifies PIKfyve as a co-therapeutic target for obinutuzumab. Clinical and translational medicine, 15(5), e70333. https://doi.org/10.1002/ctm2.70333
Ahn, T., Lee, D. H., Kim, G., Kim, J., Park, J. S., Cho, H. J., & Kim, J. Y.* (2024). Non-Invasive Strategy: Developing a Topical IL-4Rα-Specific Nanobody for the Treatment of Allergic Airway Diseases. Materials Today Bio, 101148. https://doi.org/10.1016/j.mtbio.2024.101148
Kwon, B. J., Cho, N. H., Ahn, T., Kim, G., Diệu, N. T. X., Kim, W. T., ... & Kim, J. Y.* (2024). Nicotiana benthamiana-derived dupilumab-scFv reaches deep into the cultured human nasal epithelial cells and inhibits CCL26 expression. Scientific Reports, 14(1), 14558. doi.org/10.1038/s41598-024-65524-0
J.R. Kim, D. Lee, Y. Kim & J.Y. Kim*. CD20/TNFR1 dual-targeting antibody enhances lysosome rupture-mediated cell death in B cell lymphoma. Cancer Immunol Immunother (2022). https://doi.org/10.1007/s00262-022-03344-9
K. Park, H. Lim, J. Kim, Y. Hwang, Y.S. Lee, S.H. Bae, H. Kim, H. Kim, S.W. Kang, J.Y. Kim, & M.S. Lee. Lysosomal Ca2+-mediated TFEB activation modulates mitophagy and functional adaptation of pancreatic β-cells to metabolic stress. Nature Communications (2022), 13(1). https://doi.org/10.1038/s41467-022-28874-9
C.E. Kang, S. Lee, T. Ahn, D.H. Seo, B.J. Ko, M. Jung, J. Lee, J.Y. Kim*, & W.T. Kim. Antibody-dependent cellular cytotoxicity-null effector developed using mammalian and plant GlycoDelete platform. Scientific Reports (2022), 12(1). https://doi.org/10.1038/s41598-022-23311-9
C.E. Kang, S. Lee, D.H. Seo, W. Heo, S.H. Kwon, J. Kim, J. Lee, B.J. Ko, H. Koiwa, W.T. Kim, J.Y. Kim*, Comparison of CD20 Binding Affinities of Rituximab Produced in Nicotiana benthamiana Leaves and Arabidopsis thaliana Callus, Molecular biotechnology (2021). 10.1007/s12033-021-00360-5
J.W. Han, W. Heo, D. Lee, C. Kang, H.Y. Kim, I. Jun, I. So, H. Hur, M.G. Lee, M. Jung, J.Y. Kim*, Plasma Membrane Localized GCaMP-MS4A12 by Orai1 Co-Expression Shows Thapsigargin- and Ca(2+)-Dependent Fluorescence Increases, Molecules and Cells 44 (2021) 223-232. 10.14348/molcells.2021.2031
H.-Y. Kim, M. Park, C. Kang, W. Heo, S.M. Yoon, J. Lee, J.Y. Kim*, O-GlcNAcylation of light chain serine 12 mediates rituximab production doubled by thiamet G, Bioprocess and biosystems engineering (2020) 10.1007/s00449-00020-02282-z. 10.1007/s00449-020-02282-z.
W. Heo, N. Jin, M.S. Park, H.Y. Kim, S.M. Yoon, J. Lee, J.Y. Kim*, STIM1 knock-down decreases the affinity of obinutuzumab for CD20 by altering CD20 localization to Triton-soluble membrane, Clinical and experimental immunology (2020) 10.1111/cei.13427. 10.1111/cei.13427.
J.W. Han, C. Kang, Y. Kim, M.G. Lee, J.Y. Kim*, Isoproterenol-induced hypertrophy of neonatal cardiac myocytes and H9c2 cell is dependent on TRPC3-regulated CaV1.2 expression, Cell Calcium (2020) 102305. https://doi.org/10.1016/j.ceca.2020.102305.
N. Jin, J.W. Lee, W. Heo, M.Y. Ryu, M.K. So, B.J. Ko, H.Y. Kim, S.M. Yoon, J. Lee, J.Y. Kim*, W.T. Kim, Low binding affinity and reduced complement-dependent cell death efficacy of ofatumumab produced using a plant system (Nicotiana benthamiana L.), Protein expression and purification 159 (2019) 34-41. 10.1016/j.pep.2019.03.004.
H.M. Lim, W. Heo, J.W. Han, M.G. Lee, J.Y. Kim*, NPP1 is responsible for potent extracellular ATP hydrolysis as NTPDase1 in primary cultured murine microglia, Purinergic signalling 14 (2018) 157-166. 10.1007/s11302-018-9601-z.
J.W. Lee, W. Heo, J. Lee, N. Jin, S.M. Yoon, K.Y. Park, E.Y. Kim, W.T. Kim, J.Y. Kim*, The B cell death function of obinutuzumab-HDEL produced in plant (Nicotiana benthamiana L.) is equivalent to obinutuzumab produced in CHO cells, PLoS One 13 (2018) e0191075. 10.1371/journal.pone.0191075.
W.Y. Chung, J.W. Han, W. Heo, M.G. Lee, J.Y. Kim*, Overexpression of WNK1 in POMC-expressing neurons reduces weigh gain via WNK4-mediated degradation of Kir6.2, Molecular and cellular biochemistry 447 (2018) 165-174. 10.1007/s11010-018-3301-4.
H. Piao, J. Kim, S.H. Noh, H.S. Kweon, J.Y. Kim, M.G. Lee, Sec16A is critical for both conventional and unconventional secretion of CFTR, Scientific reports 7 (2017) 39887. 10.1038/srep39887.
H.M. Lim, H. Woon, J.W. Han, Y. Baba, T. Kurosaki, M.G. Lee, J.Y. Kim*, UDP-Induced Phagocytosis and ATP-Stimulated Chemotactic Migration Are Impaired in STIM1-/- Microglia In Vitro and In Vivo, Mediators of inflammation 2017 (2017) 8158514. 10.1155/2017/8158514.
H.J. Cho, W. Heo, J.W. Han, Y.H. Lee, J.M. Park, M.J. Kang, J.H. Yoon, M.G. Lee, C.H. Kim, J.Y. Kim*, Chronological change of right ventricle by chronic intermittent hypoxia in mice, Sleep (2017). 10.1093/sleep/zsx103.
J. Kim, S.H. Noh, H. Piao, D.H. Kim, K. Kim, J.S. Cha, W.Y. Chung, H.S. Cho, J.Y. Kim, M.G. Lee, Monomerization and ER Relocalization of GRASP Is a Requisite for Unconventional Secretion of CFTR, Traffic (Copenhagen, Denmark) (2016). 10.1111/tra.12403.
J.W. Han, Y.H. Lee, S.I. Yoen, J. Abramowitz, L. Birnbaumer, M.G. Lee, J.Y. Kim*, Resistance to pathologic cardiac hypertrophy and reduced expression of CaV1.2 in Trpc3-depleted mice, Molecular and cellular biochemistry 421 (2016) 55-65. 10.1007/s11010-016-2784-0.
W.Y. Chung, M. Song, J. Park, W. Namkung, J. Lee, H. Kim, M.G. Lee, J.Y. Kim*, Generation of DeltaF508-CFTR T84 cell lines by CRISPR/Cas9-mediated genome editing, Biotechnology letters 38 (2016) 2023-2034. 10.1007/s10529-016-2190-4.
D.K. Heo, H.M. Lim, J.H. Nam, M.G. Lee, J.Y. Kim*, Regulation of phagocytosis and cytokine secretion by store-operated calcium entry in primary isolated murine microglia, Cellular signalling 27 (2015) 177-186. 10.1016/j.cellsig.2014.11.003.
H.Y. Gee, J.Y. Kim, M.G. Lee, Analysis of conventional and unconventional trafficking of CFTR and other membrane proteins, Methods Mol Biol 1270 (2015) 137-154. 10.1007/978-1-4939-2309-0_11.
S.I. Yeon, J.Y. Kim, D.S. Yeon, J. Abramowitz, L. Birnbaumer, S. Muallem, Y.H. Lee, Transient receptor potential canonical type 3 channels control the vascular contractility of mouse mesenteric arteries, PLoS One 9 (2014) e110413. 10.1371/journal.pone.0110413.
W.Y. Chung, H.W. Park, J.W. Han, M.G. Lee, J.Y. Kim*, WNK4 inhibits plasma membrane targeting of NCC through regulation of syntaxin13 SNARE formation, Cellular signalling 25 (2013) 2469-2477. 10.1016/j.cellsig.2013.08.006.
J.H. Lee, J.H. Nam, J. Park, D.W. Kang, J.Y. Kim, M.G. Lee, J.S. Yoon, Regulation of SLC26A3 activity by NHERF4 PDZ-mediated interaction, Cellular signalling 24 (2012) 1821-1830. 10.1016/j.cellsig.2012.05.010.
D.K. Heo, W.Y. Chung, H.W. Park, J.P. Yuan, M.G. Lee, J.Y. Kim*, Opposite regulatory effects of TRPC1 and TRPC5 on neurite outgrowth in PC12 cells, Cellular signalling 24 (2012) 899-906. 10.1016/j.cellsig.2011.12.011.
H.W. Park, J.Y. Kim, S.K. Choi, Y.H. Lee, W. Zeng, K.H. Kim, S. Muallem, M.G. Lee, Serine-threonine kinase with-no-lysine 4 (WNK4) controls blood pressure via transient receptor potential canonical 3 (TRPC3) in the vasculature, Proc Natl Acad Sci U S A 108 (2011) 10750-10755. 10.1073/pnas.1104271108.
W.Y. Chung, H.W. Park, D.K. Heo, K.H. Kim, M.G. Lee, J.Y. Kim*, WNK4 kinase negatively regulates the surface expression of muscarinic M3 receptor, Cellular signalling 23 (2011) 566-571. 10.1016/j.cellsig.2010.11.008.
H.W. Park, J.H. Nam, J.Y. Kim, W. Namkung, J.S. Yoon, J.S. Lee, K.S. Kim, V. Venglovecz, M.A. Gray, K.H. Kim, M.G. Lee, Dynamic regulation of CFTR bicarbonate permeability by [Cl-]i and its role in pancreatic bicarbonate secretion, Gastroenterology 139 (2010) 620-631. 10.1053/j.gastro.2010.04.004.
J.S. Lee, Y.M. Lee, J.Y. Kim, H.W. Park, S. Grinstein, J. Orlowski, E. Kim, K.H. Kim, M.G. Lee, BetaPix up-regulates Na+/H+ exchanger 3 through a Shank2-mediated protein-protein interaction, J Biol Chem 285 (2010) 8104-8113. 10.1074/jbc.M109.055079.
J. Kim, W. Namkung, J.S. Yoon, M.J. Jo, S.H. Lee, K.H. Kim, J.Y. Kim*, M.G. Lee, The role of translation elongation factor eEF1A in intracellular alkalinization-induced tumor cell growth, Lab Invest 89 (2009) 867-874. 10.1038/labinvest.2009.53.
H.Y. Gee, Y.W. Kim, M.J. Jo, W. Namkung, J.Y. Kim, H.W. Park, K.S. Kim, H. Kim, A. Baba, J. Yang, E. Kim, K.H. Kim, M.G. Lee, Synaptic scaffolding molecule binds to and regulates vasoactive intestinal polypeptide type-1 receptor in epithelial cells, Gastroenterology 137 (2009) 607-617, 617.e601-604. 10.1053/j.gastro.2009.01.065
P.F. Worley, W. Zeng, G.N. Huang, J.P. Yuan, J.Y. Kim, M.G. Lee, S. Muallem, TRPC channels as STIM1-regulated store-operated channels, Cell Calcium 42 (2007) 205-211. 10.1016/j.ceca.2007.03.004.
P.F. Worley, W. Zeng, G. Huang, J.Y. Kim, D.M. Shin, M.S. Kim, J.P. Yuan, K. Kiselyov, S. Muallem, Homer proteins in Ca2+ signaling by excitable and non-excitable cells, Cell Calcium 42 (2007) 363-371. 10.1016/j.ceca.2007.05.007.
G. Huang, J.Y. Kim, M. Dehoff, Y. Mizuno, K.E. Kamm, P.F. Worley, S. Muallem, W. Zeng, Ca2+ signaling in microdomains: Homer1 mediates the interaction between RyR2 and Cav1.2 to regulate excitation-contraction coupling, J Biol Chem 282 (2007) 14283-14290. 10.1074/jbc.M611529200.
J.Y. Kim*, W. Zeng, K. Kiselyov, J.P. Yuan, M.H. Dehoff, K. Mikoshiba, P.F. Worley, S. Muallem, Homer 1 mediates store- and inositol 1,4,5-trisphosphate receptor-dependent translocation and retrieval of TRPC3 to the plasma membrane, J Biol Chem 281 (2006) 32540-32549. 10.1074/jbc.M602496200.
G.N. Huang, W. Zeng, J.Y. Kim, J.P. Yuan, L. Han, S. Muallem, P.F. Worley, STIM1 carboxyl-terminus activates native SOC, I(crac) and TRPC1 channels, Nat Cell Biol 8 (2006) 1003-1010. 10.1038/ncb1454.
K. Kiselyov, J.Y. Kim, W. Zeng, S. Muallem, Protein-protein interaction and functionTRPC channels, Pflugers Arch 451 (2005) 116-124. 10.1007/s00424-005-1442-2.
J.Y. Kim, W. Han, W. Namkung, J.H. Lee, K.H. Kim, H. Shin, E. Kim, M.G. Lee, Inhibitory regulation of cystic fibrosis transmembrane conductance regulator anion-transporting activities by Shank2, J Biol Chem 279 (2004) 10389-10396. 10.1074/jbc.M312871200.
M. Park, S.B. Ko, J.Y. Choi, G. Muallem, P.J. Thomas, A. Pushkin, M.S. Lee, J.Y. Kim, M.G. Lee, S. Muallem, I. Kurtz, The cystic fibrosis transmembrane conductance regulator interacts with and regulates the activity of the HCO3- salvage transporter human Na+-HCO3- cotransport isoform 3, J Biol Chem 277 (2002) 50503-50509. 10.1074/jbc.M201862200.
S.B. Ko, N. Shcheynikov, J.Y. Choi, X. Luo, K. Ishibashi, P.J. Thomas, J.Y. Kim, K.H. Kim, M.G. Lee, S. Naruse, S. Muallem, A molecular mechanism for aberrant CFTR-dependent HCO(3)(-) transport in cystic fibrosis, Embo j 21 (2002) 5662-5672. 10.1093/emboj/cdf580
J.Y. Kim, K.H. Kim, J.A. Lee, W. Namkung, A.Q. Sun, M. Ananthanarayanan, F.J. Suchy, D.M. Shin, S. Muallem, M.G. Lee, Transporter-mediated bile acid uptake causes Ca2+-dependent cell death in rat pancreatic acinar cells, Gastroenterology 122 (2002) 1941-1953. https://doi.org/10.1053/gast.2002.33617
M.G. Lee, W. Ahn, J.A. Lee, J.Y. Kim, J.Y. Choi, O.W. Moe, S.L. Milgram, S. Muallem, K.H. Kim, Coordination of pancreatic HCO3- secretion by protein-protein interaction between membrane transporters, JOP : Journal of the pancreas 2 (2001) 203-206. View
W. Ahn, K.H. Kim, J.A. Lee, J.Y. Kim, J.Y. Choi, O.W. Moe, S.L. Milgram, S. Muallem, M.G. Lee, Regulatory interaction between the cystic fibrosis transmembrane conductance regulator and HCO3- salvage mechanisms in model systems and the mouse pancreatic duct, J Biol Chem 276 (2001) 17236-17243. 10.1074/jbc.M011763200.